Evaluation of Nitric Oxide and Some Biochemical Changes in Type 2 Diabetic Patients in Hilla City
Mufeed Jalil Ewadh,
Nibras Yahya Al-Khafaji
Issue:
Volume 2, Issue 4, August 2014
Pages:
50-54
Received:
18 July 2014
Accepted:
28 July 2014
Published:
10 August 2014
Abstract: The study was conducted on fifty seven of type 2 diabetic patients and thirty one apparently healthy control subject. Blood samples obtained from all subjects from Marjan Medical City and AL-Hilla Teaching Hospital in Babylon Province. The aim of this study to evaluate the differences of oxidant (nitric oxide and malonyldialdehyde), antioxidant enzyme (superoxide dismutase), trace elements (copper and zinc) and lipid profile in sera of type 2 diabetic patients and the control group. The results of present study reveal a significant increase in nitric oxide, malonyldialdehyde, copper, glucose, total cholesterol, TG, VLDL-cholesterol and LDL-cholesterol concentration in sera of type 2 diabetic patients when compared to those of the control group. Also this study show significant decrease in superoxide dismutase and zinc concentration in sera of type 2 diabetic patients when compared to those of the control group. The study concluded that type 2 diabetic patients is associated with increase oxidative stress. The increase of nitric oxide, malonyldialdehyde and copper concentration and decrease of super oxide dismutase and zinc concentration may contribute in the development of complications in diabetic patients.
Abstract: The study was conducted on fifty seven of type 2 diabetic patients and thirty one apparently healthy control subject. Blood samples obtained from all subjects from Marjan Medical City and AL-Hilla Teaching Hospital in Babylon Province. The aim of this study to evaluate the differences of oxidant (nitric oxide and malonyldialdehyde), antioxidant enz...
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Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity
Hassan Zanna,
Sa’idu Ahmad,
Babagana Abdulmalik,
Magaji Tasi’u,
Grace Okonkwo Abel,
Hauwa Mustapha Musa
Issue:
Volume 2, Issue 4, August 2014
Pages:
55-59
Received:
28 July 2014
Accepted:
11 August 2014
Published:
20 August 2014
Abstract: The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia doka and Momordica charantia Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.
Abstract: The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60...
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