Antioxidant, Antiquorum-Sensing and Antibiofilm Activities of Balanites aegyptiaca (L.) Del. (Balanitaceae) and Terminalia macroptera Guill. and Perr. (Combretaceae)
Vincent Ouedraogo,
Ablassé Rouamba,
Eli Compaoré,
Moussa Compaoré,
Martin Kiendrebeogo
Issue:
Volume 6, Issue 4, August 2018
Pages:
26-31
Received:
24 August 2018
Accepted:
11 September 2018
Published:
11 October 2018
Abstract: Pseudomonas aeruginosa like many pathogen bacteria produces various virulence factors and form biofilm that contribute to its pathogenicity and the growing resistance to antibiotics. The production of virulence factors in those multi-resistant bacteria is controlled by a mechanism of regulation termed quorum sensing. Interfering with this mechanism of bacterial communication constitute a strategy to attenuate bacterial pathogenicity. Our study aimed to assess the ability of Balanites aegyptiaca and Terminalia macroptera to interfere with the system of QS through inhibition of QS-controlled factors production and the formation of biofilm. Methanol extracts of galls, stem bark of B. aegyptiaca, and stem bark of T. macroptera were screened for anti-QS activity using Chromobacterium violaceum CV026 and P. aeruginosa PAO1. At a sub-inhibitory concentration of 100 µg/mL, galls and stem bark of B. aegyptiaca quenched the QS system by inhibiting violacein production on C. violaceum CV026 and pyocyanin production on P. aeruginosa PAO1. The bark of T. macroptera reduced significantly the production of violacein, pyocyanin and the formation of biofilm. Moreover, antioxidant activity of phenolic compounds contributes to reduce the oxidative stress induced by pyocyanin. Thus, T. macroptera is a potential candidate for the identification and isolation of news effective anti-QS compounds. This study introduces a possible validation for traditional use of B. aegyptiaca and T. macroptera and constitutes a new therapeutic approach for the treatment of infections caused by bacteria resistant to antibiotics.
Abstract: Pseudomonas aeruginosa like many pathogen bacteria produces various virulence factors and form biofilm that contribute to its pathogenicity and the growing resistance to antibiotics. The production of virulence factors in those multi-resistant bacteria is controlled by a mechanism of regulation termed quorum sensing. Interfering with this mechanism...
Show More
Phytochemical Screening, Chemical Composition and Antioxidant Activity of Leaves and Bark Extracts from Khaya senegalensis
Marvit Osman Widdat Allah,
Ayat Ahmed Alrasheid,
Altayeb Suliman Elamin
Issue:
Volume 6, Issue 4, August 2018
Pages:
32-38
Received:
5 September 2018
Accepted:
30 September 2018
Published:
1 November 2018
Abstract: Khaya senegalensis belonging to family Meliaceae. This plant has been used as traditional medicine for several disease such as pain, inflammation, malaria, anemia, diabetic and gastrointestinal diseases. The objective of this study to carry out phytochemical screening, determination of chemical composition and determine antioxidant activity of plant extracts. Many of secondary metabolites were investigated in leaves extract include alkaloids, flavonoids, sterols and saponins while the bark extract includes sterols, triterpines, tannins and saponins. The antioxidant potential of ethanol extract of leaves and bark was determined on the basis of their scavenging activity of the stable 1, 1-diphenyl-2-picryl hydrazyl (DPPH) free radical. Antioxidant activity of leaves extract was higher than bark with (42.69%) and (36.36%) respectively. The quantitative analysis of chemical composition was determined by Gas Chromatography–Mass Spectrometry (GC-MS). A number of bioactive compounds were present in the leaves and bark of K.senegalensis. The highest compound in both extracts represented the reducing sugar 4-O-Methyl mannose (66.47 and 14.73%) in bark and leaves respectively. Fatty acids and phenols were presented in high percentage in both extract include; n-Hexadecanoic acid, 9,12,15-Octadecatrienoic acid, Oleic Acid and Catechol.
Abstract: Khaya senegalensis belonging to family Meliaceae. This plant has been used as traditional medicine for several disease such as pain, inflammation, malaria, anemia, diabetic and gastrointestinal diseases. The objective of this study to carry out phytochemical screening, determination of chemical composition and determine antioxidant activity of plan...
Show More
