Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of <i>Pleiocarpa mutica </i>Leaves

Osmund Chukwuma Enechi; Christian Chijioke Amah; Jacob Ikechukwu Okoro; Ursula Chidimma Obelenwa; Ernest Chinecherem Omeje; Emmanuella Nkechinyere Osondu; Chidimma Faith Ejikeme; Chidubem Francis Okoye; Sebastine Obiajulu Maduneme; Christabel Ebube Festus; Nnadike Henry Okafor

doi:doi:10.11648/j.ab.20221001.14

Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves

Osmund Chukwuma Enechi, Christian Chijioke Amah, Jacob Ikechukwu Okoro, Ursula Chidimma Obelenwa, Ernest Chinecherem Omeje, Emmanuella Nkechinyere Osondu, Chidimma Faith Ejikeme, Chidubem Francis Okoye, Sebastine Obiajulu Maduneme, Christabel Ebube Festus, Nnadike Henry Okafor

Published in Advances in Biochemistry (Volume 10, Issue 1)

Received: 16 December 2021 Accepted: 11 January 2022 Published: 9 February 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Pleiocarpa mutica is a medicinal plant that is native to large areas of tropical Africa and belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders. The present study investigated the anti-inflammatory and anti-oxidant effects of flavonoid-rich fraction of Pleiocarpa mutica leaves (FRFPML) using both in-vivo and in-vitro based assays. A total of thirty (30) adult male albino rats (110-150g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 untreated; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 200, 400 and 600 mg/kg body weight of FRFPML respectively. Tannins, phenols, flavonoids, alkaloids, steroids, terpenoids and saponins were found in varied amounts in the plant secondary metabolites according to quantitative analysis. The extract demonstrated no harm in an acute toxicity assay when dosed up to 5000 mg/kg b.w. Scalar dosages of the FRFPML significantly (p<0.05) suppressed the development of paw oedema induced by egg albumin in the systemic rat paw oedema model. This compares favorably to the standard anti–inflammatory drug indomethacin (10 mg/kg b.w) which at 24 hours inhibited egg albumin-induced rat paw oedema (11.82%). Varying doses of the extract significantly (p<0.05) inhibited egg albumin denaturation, hypotonicity-induced membrane stabilization, protease inhibition and phospholipase A₂ in a concentration-dependent manner, causing inhibition comparable to that of the standard anti-inflammatory drug used. The extract at different concentrations (0.2-0.8 mg/ml), significantly (p<0.05) exhibited DPPH (1-1diphenyl-2-picrylhydrazyl) and H₂O₂ (hydrogen peroxide) radical scavenging activities in a concentration-dependent manner similar to the standard antioxidant ascorbic acid used. Results from the study revealed that the FRFPML exhibited remarkable anti-inflammatory and anti-oxidant activity.

Published in	Advances in Biochemistry (Volume 10, Issue 1)
DOI	10.11648/j.ab.20221001.14
Page(s)	25-34
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Pleiocarpa mutica, Anti-inflammation, Paw Odema, Anti-oxidant

References

[1]	Anand U., Jacobo-Herrera N., Altemimi A., and Lakhssassi N. (2019) A Comprehensive Review on Medicinal Plants as Antimicrobial Therapeutics: Potential Avenues of Biocompatible Drug Discovery. Metabolites, 9, 258. doi: 10.3390/metabo9110258.
[2]	Tasneem S., Liu B., Li B., Choudhary M. I., and Wang W. (2018) Molecular pharmacology of inflammation: Medicinal plants as anti-Inflammatory agents. Pharmacological Research, 139, 126-140.
[3]	Kunle O. F., Egharevba H. O., and Ahmadu P. O., (2012) Standardization of herbal medicines-A review. International Journal of Biodiversity Conservation, 4, 101–112.
[4]	Pye C. R., Bertin M. J., Lokey R. S., Gerwick W. H., and Linington R. G. (2017) Retrospective analysis of natural products provides insights for future discovery trends. Proceedings National Academy of Sciences of the USA, 114, 5601–5606.
[5]	Sultana N., and Saify Z. S. (2012) Naturally occurring and synthetic agents as potential anti-inflammatory and immunomodulants. Anti-Inflammatory Anti-Allergy Agents in Medicinal Chemistry, 11, 3–19.
[6]	Orlikova B., Legrand N., Panning J., Dicato M., and Diederich M. (2014) Anti-inflammatory and anticancer drugs from nature. Cancer Treatment and Research Communications, 159, 123–143.
[7]	Okeke E. S., Enechi O. C., Nwankwo N. E., and Nwodo O. F. C. (2019) Evaluation of the Phytochemical Constituents and Anti-Inflammatory Potential of FagaraZanthoxyloides Root-Bark Using In Vivo and In Vitro Models. Pharmacology Online, 2, 212–224.
[8]	Chandra S., Chatterjee P., Dey P., and Bhattacharya S. (2012) Evaluation of in vitro anti-inflammatory activity of coffee against the denaturation of the protein. Asian Pacific Journal of Tropical Biomedicine, 2, 178–180.
[9]	Joseph, L., and M. George (2016) Evaluation of in vivo and in vitro anti-inflammatory activity of novel isoxazoleseries. European International Journal of Science and Technology, 5 (3), 35-42.
[10]	Chaitanya R., Sandhya S., David B., Vinod K. R. and Murali S. (2011) HRBC Membrane Stabilizing Property of Roor, Stem and Leaf of Glochidionvelutinum. International Journal of Research in Pharmaceutical Biomedical Science, 2 (1), 256–259.
[11]	Anosike C. A., Obidoa O. and Ezeanyika L. U. S. (2012) Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU Journal of Pharmaceutical Sciences, 20, 76. doi: 10.1186/2008-2231-20-76.
[12]	Ullah H. A., Zaman S., Juhara F., Akter L., Tareq S. M., Masum E. H. and Bhattacharjee R. (2014) Evaluation of antinociceptive, in-vivo & in-vitro anti-inflammatory activity of ethanolic extract of Curcuma zedoaria rhizome. BMC complementary and alternative medicine, 14 (1), 346.
[13]	Markiewski M. M. and Lambris J. D. (2007) The role of complement in inflammatory diseases from behind the scenes into the spotlight. American Journal of Pathology, 171 (3), 715–727.
[14]	Leelaprakash G. and Dass S. M. (2011) In vitro anti-inflammatory activity of methanol extract of Enicostemmaaxillare. International Journal of Drug Development Research, 3, 189–196.
[15]	Coussens L. M. and Werb Z. (2002) Inflammation and cancer. Nature, 420, 860–867.
[16]	Reuter S., Gupta S. C., Chaturvedi M. M. and Aggarwal B. B. (2010) Oxidative stress, inflammation, and cancer: How are they linked? Free Radical Biology and Medicine, 49, 1603–1616.
[17]	Arulselvan P., Fard M. T., Tan W. S., Gothai S., Fakurazi S., Norhaizan M. E. and Kumar S. S. (2016) Role of Antioxidants and Natural Products in Inflammation. Oxidative Medicine and Cellular Longevity, 2016, 1-15.
[18]	Omoyeni O. A., Hussein A. A., Iwuoha E. and Green I. R. (2017) A review of the ethnomedicinal uses, phytochemistryand pharmacology of the Pleiocarpa genus. Phytochemistry Reviews, 16, 97-115.
[19]	TPL The Plant List (2013) Version 1. Published on the Internet. http://www.theplantlist.org/, 2013. Accessed 10 December, 2021.
[20]	Schmelzer G. H. (2008) Medicinal plants, PROTA, 2008.
[21]	Burkill H. M. (1985) “The useful plants of West Tropical Africa, families A-D”. Royal Botanic Gardens, Kew.
[22]	Quattrocchi U. (2012) “CRC world dictionary of medicinal and poisonous plants: common names, scientific names, eponyms, synonyms and etymology”. CRC Press, Boca Raton.
[23]	Hermans M., Akoe`gninou A. and van der Maesen J. (2004) Medicinal plants used to treat malaria in southern Benin. Economic Botany, 58, 239–252.
[24]	Addae-Kyereme J., Croft S. L, Kendrick H. and Wright C. W. (2001) Antiplasmodial activities of some Ghanaian plants traditionally used for fever/malaria treatment and of some alkaloids isolated from Pleiocarpa mutica; in-vivo antimalarial activity of pleiocarpine. Journal of Ethnopharmacology, 76, 99–103.
[25]	Enechi O. C., Okenu B. C., and Uroko R. I. (2016) Antimalarial Activity and Effect of Ethanol Extract of Pleiocarpamutica Leaves on Some Haematological Indices of Plasmodium berghei-Infected Mice. American-Eurasian Journal of Agriculture and Environmental Science, 16 (5), 860-867.
[26]	Derrell C. (1996) Guide for care and use of laboratory animals. Institute of Laboratory Animal Resources. National Academy Press, Washington DC.
[27]	Harborne J. B. (1998) “Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis”, 2^nd Ed, London: Chapman and Hall Limited.
[28]	Chu Y. F., Sun J., Wu X., and Liu R. H. (2002) Anti-oxidant and anti-proliferation activities of common vegetables. Journal of Agriculture and Food Chemistry, 50 (23), 6910–6.
[29]	Lorke D. (1983) A new approach to practical acute toxicity testing. Archives of Toxicology, 54 (4), 275–87.
[30]	Winter C. A., Risley E. A., and Nuss G. W. (1962) Carrageenin-induced oedema in hind paw of the rats as an assay for anti-inflammatory drugs. Proceedings of the Society for Experimental Biology and Medicine, 111 (3), 544–7.
[31]	Haghparast M. R., Ali C., Khosroyar S. and Mohammadi A. (2018) Evaluation of in Vitro Anti-Inflammatory Activity of Harpagophytumprocumbens and Urticadioica Against the Denaturation of Protein. Plant Archives, 18 (1), 161-166.
[32]	Oyedapo O. O. and Famurewa A. J. (1995) Antiprotease and membrane stabilizing activities of extracts of Fagarazanthoxyloides, Olaxsubscorpioides and Tetrapleuratetraptera. International Journal of Pharmacology, 33, 65–69.
[33]	Chou C. (1997) The Antiinflammatory effect of an extract of Tripterygium wilfordii hook F on adjuvant-induced paw Oedema in rats and Inflammatory mediators release. Journal of Medicinal Plant Research, 11, 152–4.
[34]	Vane J. R. (1971) Inhibition of prostaglandin synthesis as a mechanism of action for aspirin-like drugs. Nature: New Biology, 231 (25), 232–235.
[35]	Enechi O. C., Okeke E. S., Awoh O. E., Okoye C. O. and Odo C. K. (2021) Inhibition of phospholipase A₂, platelet aggregation and egg albumin induced rat paw oedema as anti-inflammatory effect of Peltophorunpterocarpus stem-bark. Clinical Phytoscience, 7, 75.
[36]	Brand-Williams W., Cuvelier M. E. and Berset C. (1995) Use of free radical method to evaluate antioxidant activity. LWT Food Science and Technology, 28, 25-30.
[37]	Ruch R. J., Cheng S. J. and Klaunig J. E. (1989) Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Journal of Carcinogenesis, 10, 1003-1008.
[38]	George A., Chinnappan S., Chintamaneni M., Kotak C. V. and Choudhary Y. (2014) Anti-inflammatory effects of Polygonum minus (Huds) extract (Lineminus) inin-vitro enzyme assays and carrageenan induced paw oedema. BMC Complementary Alternative Medicine, 14, 1-7.
[39]	Sakat S., Juvekar A. R. and Gambhire M. N. (2010) In vitro antioxidant and antiinflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharmaceutical Science, 2 (1), 146–55.
[40]	Maleki S. J., Crespob J. F. and Cabanillas B. (2019) Anti-inflammatory effects of flavonoids. Food Chemistry, 299, 124-125.
[41]	Karthik K., Kumar B. R., Priya K. R. V., Kumar S. K. and Rathore R. S. B. (2013) Evaluation of anti-inflammatory activity of canthium parviflorum by in-vitro method. Indian Journal of Research in Pharmacy and Biotechnology, 5674, 2320–2322.
[42]	Gunathilake K. D. P. P., Ranaweera K. K. D. S. and Rupasinghe H. P. V. (2018) In Vitro Anti-Inflammatory Properties of Selected Green Leafy Vegetables. Biomedicines, 6, 107. doi: 10.3390/biomedicines6040107.
[43]	FeirraliM., Signormi C., Ciccolili L. and Comporti M. (1992) Iron release and membranedamage in erythrocytes exposed to oxidizing agents, phenylhydrazine, devicene and iso-uranil. Biochemical Journal, 285, 295–301.
[44]	Halliwell B., and Whiteman M. Measuring reactive species and oxidativedamage in vivo and in cell culture: how should you do it and what do the results mean? British Journal of Pharmacology, 142 (2), 231–225.
[45]	Govindappa M., Naga S. S., Poojashri M. N., Sadananda T. S. and Chandrappa C. P. (2011) Antimicrobial, antioxidant and in vitro anti-inflammatory activity of ethanol extract and active phytochemical screening of Wedeliatrilobata (L.) Hitchc. Journal of Pharmacognosy and Phytotherapy, 3, 43–51.
[46]	Sato H., Taketomi Y., Isogai Y., Masuda S. and Kobayashi T. (2009) Group III secreted phospholipase A₂ transgenic mice spontaneously develop inflammation. Biochemical Journal, 421, 17-27.
[47]	Kim H. P., Park H., Son K. H., Chang H. W. and Kang S. S. (2008) Biochemical pharmacology of biflavonoids: Implications for anti-inflammatory action. Archives of Pharmacal Research, 31, 265-273.
[48]	Mittal M., Siddiqui M. R., Tran K., Reddy S. P. and Malik A. B. (2014) Reactive oxygen species in inflammation and tissue injury. Antioxidants & Redox Signaling, 20, 1126–1167.
[49]	Nimse S. B. and Pal D. (2015). Free radicals, natural antioxidants, and their reaction mechanisms. Rsc Advances, 5, 27986–28006.
[50]	Li X., Jiang Q., Wang T., Liu J., and Chen D. (2016). Comparison of the antioxidant effects of quercitrin and isoquercitrin: Understanding the role of the 6 ″-OH group. Molecules, 21.
[51]	Chen G. L., Fan M. X., Wu J. L., Li N. and Guo M. Q. (2019). Antioxidant and anti-inflammatory properties of flavonoids from lotus plumule. Food Chemistry, 277, 706–712.

Cite This Article

Plain Text BibTeX RIS

APA Style

Osmund Chukwuma Enechi, Christian Chijioke Amah, Jacob Ikechukwu Okoro, Ursula Chidimma Obelenwa, Ernest Chinecherem Omeje, et al. (2022). Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Advances in Biochemistry, 10(1), 25-34. https://doi.org/10.11648/j.ab.20221001.14

Copy | Download

ACS Style

Osmund Chukwuma Enechi; Christian Chijioke Amah; Jacob Ikechukwu Okoro; Ursula Chidimma Obelenwa; Ernest Chinecherem Omeje, et al. Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Adv. Biochem. 2022, 10(1), 25-34. doi: 10.11648/j.ab.20221001.14

Copy | Download

AMA Style

Osmund Chukwuma Enechi, Christian Chijioke Amah, Jacob Ikechukwu Okoro, Ursula Chidimma Obelenwa, Ernest Chinecherem Omeje, et al. Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Adv Biochem. 2022;10(1):25-34. doi: 10.11648/j.ab.20221001.14

Copy | Download

@article{10.11648/j.ab.20221001.14,
  author = {Osmund Chukwuma Enechi and Christian Chijioke Amah and Jacob Ikechukwu Okoro and Ursula Chidimma Obelenwa and Ernest Chinecherem Omeje and Emmanuella Nkechinyere Osondu and Chidimma Faith Ejikeme and Chidubem Francis Okoye and Sebastine Obiajulu Maduneme and Christabel Ebube Festus and Nnadike Henry Okafor},
  title = {Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves},
  journal = {Advances in Biochemistry},
  volume = {10},
  number = {1},
  pages = {25-34},
  doi = {10.11648/j.ab.20221001.14},
  url = {https://doi.org/10.11648/j.ab.20221001.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20221001.14},
  abstract = {Pleiocarpa mutica is a medicinal plant that is native to large areas of tropical Africa and belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders. The present study investigated the anti-inflammatory and anti-oxidant effects of flavonoid-rich fraction of Pleiocarpa mutica leaves (FRFPML) using both in-vivo and in-vitro based assays. A total of thirty (30) adult male albino rats (110-150g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 untreated; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 200, 400 and 600 mg/kg body weight of FRFPML respectively. Tannins, phenols, flavonoids, alkaloids, steroids, terpenoids and saponins were found in varied amounts in the plant secondary metabolites according to quantitative analysis. The extract demonstrated no harm in an acute toxicity assay when dosed up to 5000 mg/kg b.w. Scalar dosages of the FRFPML significantly (p2 in a concentration-dependent manner, causing inhibition comparable to that of the standard anti-inflammatory drug used. The extract at different concentrations (0.2-0.8 mg/ml), significantly (p2O2 (hydrogen peroxide) radical scavenging activities in a concentration-dependent manner similar to the standard antioxidant ascorbic acid used. Results from the study revealed that the FRFPML exhibited remarkable anti-inflammatory and anti-oxidant activity.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves
AU  - Osmund Chukwuma Enechi
AU  - Christian Chijioke Amah
AU  - Jacob Ikechukwu Okoro
AU  - Ursula Chidimma Obelenwa
AU  - Ernest Chinecherem Omeje
AU  - Emmanuella Nkechinyere Osondu
AU  - Chidimma Faith Ejikeme
AU  - Chidubem Francis Okoye
AU  - Sebastine Obiajulu Maduneme
AU  - Christabel Ebube Festus
AU  - Nnadike Henry Okafor
Y1  - 2022/02/09
PY  - 2022
N1  - https://doi.org/10.11648/j.ab.20221001.14
DO  - 10.11648/j.ab.20221001.14
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 25
EP  - 34
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20221001.14
AB  - Pleiocarpa mutica is a medicinal plant that is native to large areas of tropical Africa and belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders. The present study investigated the anti-inflammatory and anti-oxidant effects of flavonoid-rich fraction of Pleiocarpa mutica leaves (FRFPML) using both in-vivo and in-vitro based assays. A total of thirty (30) adult male albino rats (110-150g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 untreated; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 200, 400 and 600 mg/kg body weight of FRFPML respectively. Tannins, phenols, flavonoids, alkaloids, steroids, terpenoids and saponins were found in varied amounts in the plant secondary metabolites according to quantitative analysis. The extract demonstrated no harm in an acute toxicity assay when dosed up to 5000 mg/kg b.w. Scalar dosages of the FRFPML significantly (p2 in a concentration-dependent manner, causing inhibition comparable to that of the standard anti-inflammatory drug used. The extract at different concentrations (0.2-0.8 mg/ml), significantly (p2O2 (hydrogen peroxide) radical scavenging activities in a concentration-dependent manner similar to the standard antioxidant ascorbic acid used. Results from the study revealed that the FRFPML exhibited remarkable anti-inflammatory and anti-oxidant activity.
VL  - 10
IS  - 1
ER  -

Copy | Download

Author Information

Osmund Chukwuma Enechi

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Christian Chijioke Amah

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Jacob Ikechukwu Okoro

Medical Parasitology, Toxicology and Drug Development Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Ursula Chidimma Obelenwa

Department of Microbiology, Alex Ekwueme Federal University, Ndufu-Alike, Nigeria
Ernest Chinecherem Omeje

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Emmanuella Nkechinyere Osondu

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Chidimma Faith Ejikeme

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Chidubem Francis Okoye

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Sebastine Obiajulu Maduneme

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Christabel Ebube Festus

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Nnadike Henry Okafor

Pharamacological Biochemistry Research Unit, Department of Biochemistry, University of Nigeria, Nsukka, Nigeria

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Osmund Chukwuma Enechi, Christian Chijioke Amah, Jacob Ikechukwu Okoro, Ursula Chidimma Obelenwa, Ernest Chinecherem Omeje, et al. (2022). Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Advances in Biochemistry, 10(1), 25-34. https://doi.org/10.11648/j.ab.20221001.14

Copy | Download

ACS Style

Osmund Chukwuma Enechi; Christian Chijioke Amah; Jacob Ikechukwu Okoro; Ursula Chidimma Obelenwa; Ernest Chinecherem Omeje, et al. Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Adv. Biochem. 2022, 10(1), 25-34. doi: 10.11648/j.ab.20221001.14

Copy | Download

AMA Style

Osmund Chukwuma Enechi, Christian Chijioke Amah, Jacob Ikechukwu Okoro, Ursula Chidimma Obelenwa, Ernest Chinecherem Omeje, et al. Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves. Adv Biochem. 2022;10(1):25-34. doi: 10.11648/j.ab.20221001.14

Copy | Download

@article{10.11648/j.ab.20221001.14,
  author = {Osmund Chukwuma Enechi and Christian Chijioke Amah and Jacob Ikechukwu Okoro and Ursula Chidimma Obelenwa and Ernest Chinecherem Omeje and Emmanuella Nkechinyere Osondu and Chidimma Faith Ejikeme and Chidubem Francis Okoye and Sebastine Obiajulu Maduneme and Christabel Ebube Festus and Nnadike Henry Okafor},
  title = {Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves},
  journal = {Advances in Biochemistry},
  volume = {10},
  number = {1},
  pages = {25-34},
  doi = {10.11648/j.ab.20221001.14},
  url = {https://doi.org/10.11648/j.ab.20221001.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20221001.14},
  abstract = {Pleiocarpa mutica is a medicinal plant that is native to large areas of tropical Africa and belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders. The present study investigated the anti-inflammatory and anti-oxidant effects of flavonoid-rich fraction of Pleiocarpa mutica leaves (FRFPML) using both in-vivo and in-vitro based assays. A total of thirty (30) adult male albino rats (110-150g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 untreated; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 200, 400 and 600 mg/kg body weight of FRFPML respectively. Tannins, phenols, flavonoids, alkaloids, steroids, terpenoids and saponins were found in varied amounts in the plant secondary metabolites according to quantitative analysis. The extract demonstrated no harm in an acute toxicity assay when dosed up to 5000 mg/kg b.w. Scalar dosages of the FRFPML significantly (p2 in a concentration-dependent manner, causing inhibition comparable to that of the standard anti-inflammatory drug used. The extract at different concentrations (0.2-0.8 mg/ml), significantly (p2O2 (hydrogen peroxide) radical scavenging activities in a concentration-dependent manner similar to the standard antioxidant ascorbic acid used. Results from the study revealed that the FRFPML exhibited remarkable anti-inflammatory and anti-oxidant activity.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Assessment of the Anti-inflammatory and Anti-oxidant Activities of Flavonoid-rich Fraction of Pleiocarpa mutica Leaves
AU  - Osmund Chukwuma Enechi
AU  - Christian Chijioke Amah
AU  - Jacob Ikechukwu Okoro
AU  - Ursula Chidimma Obelenwa
AU  - Ernest Chinecherem Omeje
AU  - Emmanuella Nkechinyere Osondu
AU  - Chidimma Faith Ejikeme
AU  - Chidubem Francis Okoye
AU  - Sebastine Obiajulu Maduneme
AU  - Christabel Ebube Festus
AU  - Nnadike Henry Okafor
Y1  - 2022/02/09
PY  - 2022
N1  - https://doi.org/10.11648/j.ab.20221001.14
DO  - 10.11648/j.ab.20221001.14
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 25
EP  - 34
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20221001.14
AB  - Pleiocarpa mutica is a medicinal plant that is native to large areas of tropical Africa and belongs to the family of Apocynaceae. It is a medicinal herb used in traditional Nigerian medicine to treat a variety of diseases, including inflammatory disorders. The present study investigated the anti-inflammatory and anti-oxidant effects of flavonoid-rich fraction of Pleiocarpa mutica leaves (FRFPML) using both in-vivo and in-vitro based assays. A total of thirty (30) adult male albino rats (110-150g) were divided into five (5) groups of six (6) rats each for the in-vivo anti-inflammatory assay. Group 1 untreated; Group 2 was given 10 mg/kg body weight of Indomethacin and Groups 3, 4 and 5 were given 200, 400 and 600 mg/kg body weight of FRFPML respectively. Tannins, phenols, flavonoids, alkaloids, steroids, terpenoids and saponins were found in varied amounts in the plant secondary metabolites according to quantitative analysis. The extract demonstrated no harm in an acute toxicity assay when dosed up to 5000 mg/kg b.w. Scalar dosages of the FRFPML significantly (p2 in a concentration-dependent manner, causing inhibition comparable to that of the standard anti-inflammatory drug used. The extract at different concentrations (0.2-0.8 mg/ml), significantly (p2O2 (hydrogen peroxide) radical scavenging activities in a concentration-dependent manner similar to the standard antioxidant ascorbic acid used. Results from the study revealed that the FRFPML exhibited remarkable anti-inflammatory and anti-oxidant activity.
VL  - 10
IS  - 1
ER  -

Copy | Download