Research Article
Performance Evaluation of the HemoTypeSCTM Rapid Screening Test for the Detection of Hemoglobin A, S, and C Variants at the Fann National University Hospital
Sene Moustapha Ndep*
,
Kandji Pape Matar,
Barry Nene Oumou Kesso,
Thioune Ndeye Mareme,
Ndiaye Siny,
Djite Moustapha,
Ndour El Malick,
Gueye-Tall Fatou,
Gueye Papa Madieye
Issue:
Volume 14, Issue 4, December 2026
Pages:
89-93
Received:
7 September 2026
Accepted:
20 September 2026
Published:
23 October 2026
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of the HemoTypeSCTM rapid test for detecting hemoglobin A, S, and C variants, in accordance with the NF EN ISO 15189 standard. A prospective analytical study was conducted at the Biochemistry-Hematology Laboratory of Fann National University Hospital Center. Patients referred for hemoglobin electrophoresis were tested using HemoTypeSCTM, with the Emmel test and capillary electrophoresis (Capillarys 3 octa, Sebia) as comparative methods. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and overall agreement were calculated with 95% confidence intervals. Among the 126 samples, 19 had normal profiles and 107 had hemoglobinopathies. Compared with the Emmel test, HemoTypeSCTM showed 100% sensitivity and specificity. Compared with capillary electrophoresis, sensitivity was 98.16%, specificity 100%, PPV 100%, and NPV 90.48%. Two discrepancies in fine typing were observed among the 107 abnormal profiles, with no missed abnormal profiles. HemoTypeSCTM is a rapid, reliable, and easy-to-use test for first-line sickle cell disease screening in resource-limited settings. Positive results should be systematically confirmed by electrophoresis.
Abstract: Hemoglobinopathies, particularly sickle cell disease, pose major public health challenges in resource-limited settings, where delayed diagnosis contributes to high morbidity and mortality. As the world's most common monogenic disorder, the vast majority of affected individuals reside in sub-Saharan Africa. This study evaluated the performance of th...
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Research Article
Therapeutic Effects of 80% Methanolic Cannabis Sativa Leaf Extract on Copper (II) Oxide-Induced Hepatorenal Toxicity in Wistar Rats
Issue:
Volume 14, Issue 4, December 2026
Pages:
94-99
Received:
15 August 2026
Accepted:
26 August 2026
Published:
29 October 2026
Abstract: This study aimed to investigate the protective effect of 80% methanolic Cannabis sativa leaf extract against copper (II) oxide (CuO)-induced hepatorenal toxicity in Wistar rats. Acute toxicity studies were conducted to determine the median lethal dose (LD₅₀) of the crude extract and CuO, followed by an in vivo assessment of the protective effect of the extract in rats exposed to CuO. The crude C. sativa leaf extract exhibited relatively low acute toxicity, with an LD₅₀ of 676.08 mg/kg, compared with 45.71 mg/kg for CuO, indicating greater acute toxicity of CuO. CuO exposure produced significant alterations in selected hepatic and renal biochemical parameters compared with the normal control group. Administration of the 80% methanolic C. sativa leaf extract following CuO exposure significantly ameliorated these alterations, with significant differences (P < 0.05) observed among the experimental groups. The observed changes in hepatic and renal biomarkers suggest that CuO exposure may impair normal hepatorenal function, whereas the improvement following extract administration indicates a potential protective effect of C. sativa. These findings suggest that 80% methanolic C. sativa leaf extract possesses ameliorative properties against CuO-induced hepatorenal toxicity in Wistar rats. The extract may therefore represent a potential source of bioactive compounds for mitigating heavy-metal-induced hepatic and renal injury. Further studies are warranted to identify the active phytochemical constituents and elucidate the molecular mechanisms underlying the observed protective effects.
Abstract: This study aimed to investigate the protective effect of 80% methanolic Cannabis sativa leaf extract against copper (II) oxide (CuO)-induced hepatorenal toxicity in Wistar rats. Acute toxicity studies were conducted to determine the median lethal dose (LD₅₀) of the crude extract and CuO, followed by an in vivo assessment of the protective effect of...
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Research Article
Biotechnology of Traditional Cold Oil Extraction from Balanites Aegyptiaca Kernels Across Three Regions of Senegal
Diao Mamoudou,
Dramé Abdoulaye*,
Faye Ibrahima,
Ndour Khady,
Faye Mada,
Sarr Cheikh Lamine
Issue:
Volume 14, Issue 4, December 2026
Pages:
100-107
Received:
25 September 2026
Accepted:
15 October 2026
Published:
31 October 2026
DOI:
10.11648/j.ab.20261404.13
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Abstract: The kernel oil of Balanites aegyptiaca Delile represents a significant yet underutilized natural resource in Senegal, possessing high nutritional and therapeutic potential that rivals common commercial edible oils. Despite its high oil content and favorable fatty acid profile, this resource remains largely undervalued in local economies where production is primarily demand-driven. This study focuses on the extraction and comparison of oil yields from Balanites aegyptiaca (L.) Del seeds harvested in three areas of Senegal. Kaolack, Louga, and Podor. Cold press oil extraction is more environment friendly than other oil extraction methods as it preserves the natural flavor, aroma, and nutritional content of the oil. Here, the cold pressing method was used to optimize oil pressing from Balanites aegyptiaca Delile kernels. The fruits were depulped, dried, and then crushed to extract the kernels. The oil was obtained by cold hydraulic pressing, a preferred method for preserving bioactive compounds. The results show significant differences in yield depending on the origin of the fruits. Kaolack had the highest yield (35%), followed by Louga (33.4%); Louga-Podor mixture (31.2%) and Podor (30.1%), respectively. These variations are influenced by factors such as climate, rainfall, soil quality, genetic variability of the fruit, kernel size, and extraction technique. The study concludes that the fruit from Kaolack is the most promising for the sustainable production of Balanites aegyptiaca (L.) Del. oil in Senegal. In addition many by-products, including oilseed cakes, were obtained, constituting a basic feed for animals.
Abstract: The kernel oil of Balanites aegyptiaca Delile represents a significant yet underutilized natural resource in Senegal, possessing high nutritional and therapeutic potential that rivals common commercial edible oils. Despite its high oil content and favorable fatty acid profile, this resource remains largely undervalued in local economies where produ...
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