Evaluation of Improved Pigeon pea (Cajanus cajan) Varieties at Adola, Guji Zone of Oromia
Teshale Jabessa,
Ketema Bekele
Issue:
Volume 9, Issue 1, March 2021
Pages:
1-5
Received:
6 January 2021
Accepted:
26 January 2021
Published:
30 January 2021
Abstract: A study was conducted with the objective to identify adaptable, high biomass, good quality and seed yield of Pegeon pea cultivars at Adola sub-site of Bore Agricultural research center. Four pegeon pea cultivars Tsigas, Degagsa-75, Belabas-27 and 16555 were tested in RCBD with three replications. The analysis of variation revealed that significant (P<0.05) differ in days to 90% maturity were observed among cultivars. Among tested cultivars Belabas 27 was significantly earlier (162 days) to maturity, while late matured cultivar was Degagsa (203 days). Analysis of variance showed highly significant differences (P<0.01) were observed among cultivars for number of primary branches per plant. Higher branch number was recorded for Tsigas cultivar (21.6) whereas the lowest branch number was obtained from cultivar Degagsa-75 (9.75). The cultivars were significant (P<0.05) differ for pod length. The long pod was recorded from cultivar 16555 (5 cm) while the short pod length was recorded from cultivar Belabas -27 (3.14 cm). Cultivars were significant (P<0.05) varied for plant height. The long plant height was measured from cultivar Degagsa-75 (159.75 cm) followed by Tsigas (104.9 cm) whereas the short pant height was obtained from cultivar Belabas-27 (78.63 cm). Significant variations (P<0.05) in biomass yield between genotypes were observed (Table 2). Superior biomass yield was produced from Tsigas cultivar (2.17 ton/ha) followed by cultivar 16555 (1.27 ton/ha) while the low biomass yield was obtained from Degagsa-75 cultivar (0.97 ton/ha). In addition to the nutritional values were promising particularly the crude protein (CP) content in cultivar Tsigas. Based up on its adaptability, high biomass yield, seed yield and good CP of cultivar Tsigas and 16555 is recommended for further promotion in the midland of Guji zone and similar agro- ecologies.
Abstract: A study was conducted with the objective to identify adaptable, high biomass, good quality and seed yield of Pegeon pea cultivars at Adola sub-site of Bore Agricultural research center. Four pegeon pea cultivars Tsigas, Degagsa-75, Belabas-27 and 16555 were tested in RCBD with three replications. The analysis of variation revealed that significant ...
Show More
Ameliorative Effect of Moringa Oleifera Biochemical Constituents on Blood Glucose Level of Streptozocin-Induced Diabetic Wistar Rats
Ibrahim Maina Hassan,
Bashir Saidu,
Ja’afaru Abdullahi Ishaq,
Ashiru Dahiru,
Nafisat Abdulazeez,
Halima Ibrahim Yusuf,
Aliyu Habeeb Tosin,
Nicholas Nathaniel Pilau,
Adamu Abdul Abubakar,
Muhammad Bashir Bello Muhammad Bashir Bello
Issue:
Volume 9, Issue 1, March 2021
Pages:
6-10
Received:
19 March 2020
Accepted:
11 May 2020
Published:
23 February 2021
Abstract: Medicinal plants are among the important source of potential therapeutic agents for diabetes. Therapeutic use of Moringa oleifera leaf extract has been practiced in management of diabetes because of its potential to decrease blood glucose and lipids concentration after ingestion. This study was conducted to evaluate the ameliorative effect of Moringa oleifera leaf extract on streptozocin induced diabetes Wistar rats. Initially, diabetes was induced by given streptozocin (STZ) intravenously followed by the treatments with different concentration of Moringa oleifera aqueous leaf extract. Fasting blood glucose level was measured using Accu-Chek glucometer after 24, 48, 72 and 97 hours post treatment. There is significance difference at 0.05 in blood glucose level between the control groups and the groups that were treated with the leaf extract after administration of STZ. Hence, this plant shows high potential to be used in the management of hyperglycaemia. The need for the toxicity study to evaluate it effect in body tissues and organs is recommended. There is need to study the phytochemical constituents to identify the bioactive lead compound with ameliorative effect.
Abstract: Medicinal plants are among the important source of potential therapeutic agents for diabetes. Therapeutic use of Moringa oleifera leaf extract has been practiced in management of diabetes because of its potential to decrease blood glucose and lipids concentration after ingestion. This study was conducted to evaluate the ameliorative effect of Morin...
Show More
Zinc(II) Chelate Binds to Fibrinogen and Its αC Region
Ralph Butkowski,
Derek King,
Perla Cortes
Issue:
Volume 9, Issue 1, March 2021
Pages:
11-17
Received:
26 February 2021
Accepted:
17 March 2021
Published:
26 March 2021
Abstract: Fibrinogen is the immediate precursor of fibrin blood clots. When fibrinogen is cleaved by thrombin, it assembles into fibrin polymers through intermolecular interactions where calcium and zinc ions may both be involved. Fibrinogen’s αC domains play a major role in fibrin polymer assembly, and they contain a Zn2+ binding sites based on peptide studies. However, Zn2+ binding to natural αC and fibrin (ogen) remains to be demonstrated. The objective of this study was to directly demonstrate Zn2+ interactions with purified fibrinogen and its αC region. Both human and bovine αC regions were investigated due to the significant differences in their protein sequences. To detect Zn2+ binding, chelated Zn2+ in the form of Zn2+nitrilotriacetic acid linked to alkaline phosphatase tracer (ZnTAP) was prepared and used in ELISA and Western blot style methods. Gel electrophoresis was used to monitor changes to fibrinogen after reaction with plasmin. Human and bovine αC regions were obtained by limited plasmin digests of fibrinogen and purified by Con-A and Zn-immobilized metal ion chromatography. The results showed ZnTAP binding to fibrinogen in solution and inhibition of binding to fibrinogen coated microplates by ELISA. Inhibition concentrations (IC50) of 0.58 μM for human fibrinogen and 0.26 μM for bovine fibrinogen were determined. Blot probes revealed ZnTAP binding to fibrinogen with disulfide bonds intact and to the Aα and Bβ chains with disulfide bonds reduced. Reduced Aα chain reaction was less than that of intact fibrinogen and the reduced Bβ chain was only weakly reactive. With plasmin treatment, ZnTAP binding decreased by ELISA in parallel with the degradation of αC region by gel electrophoresis analysis. Purified αC bound ZnTAP in ELISA and on Western blots. In this study we demonstrated that Zn2+ binds to fibrinogen and its Aα and Bβ chains, and to the αC region of the Aα chain. The results support a role for protein conformation in Zn2+ binding and demonstrate the utility of the ZnTAP complex with tracer for fibrinogen binding interactions.
Abstract: Fibrinogen is the immediate precursor of fibrin blood clots. When fibrinogen is cleaved by thrombin, it assembles into fibrin polymers through intermolecular interactions where calcium and zinc ions may both be involved. Fibrinogen’s αC domains play a major role in fibrin polymer assembly, and they contain a Zn2+ binding sites based on peptide stud...
Show More
