Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus

Abdulqader Wael Rasheid; Firas Taher Maher; Akeel Hussein Al-Aisse; Adnan Fadhel Nasseef

doi:doi:10.11648/j.ab.20210903.17

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Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus

Abdulqader Wael Rasheid, Firas Taher Maher, Akeel Hussein Al-Aisse, Adnan Fadhel Nasseef

Published in Advances in Biochemistry (Volume 9, Issue 3)

Received: 19 July 2021 Accepted: 7 August 2021 Published: 31 August 2021

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Abstract

Diabetes mellitus is a metabolic disease belongs to a group of disparate diseases clinically and genetically characterized by increased blood sugar (hyperglycemia) as a result of defect in insulin metabolism. Glucose-6-Phosphate Dehydrogenase (G6PD) (Oxidoreductase, EC 1.1.1.1-49) is one of the most important enzymes of the metabolite, the main enzyme and the key to the Pentose phosphate path way. Unsuitable control of blood glucose decreases G6PD activity and increases diabetes mellitus complications. the This study evaluated effect of Mediterranean mutation which causes decrease G6PD activity on diabetes patients and evaluated the difference of G6PD activity among diabetic and non-diabetic patients, and the impact of hyperglycemia on the G6PD activity and different in G6PD activity in six and age and relation between Calcium and Magnesium with G6PD activity and Mediterranean mutation and found correlative coefficient(r) between G6PD activity in Patient and Blood sugar, Calcium, Magnesium and G6PD activity in healthy people. Asixteen diabetic and non-diabetic subjects were selected from patients 8 to 60 years old. Demographic data including gender, age, height, weight, duration of diabetes mellitus, type and duration of treatment, medical history (especially fauvism) were recorded. Blood pressure and body mass index were also measured. One blood sample was taken from each subject and 5 elements including G6PD presence and activity, fasting plasma glucose, plasma Calcium, Magnesium and DNA was extraction from whole blood and then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme MboII to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). G6PD activity was significantly higher in non-diabetic subjects (P<0.05). Within diabetics, G6PD mean activity was significantly higher in non-group (P<0.05) and in subjects with BMI < 25 (P<0.05). G6PD mean activity was significantly higher in non-diabetics than (P<0.01) and Ca, Mg diabetics patients (P<0.05) the Med-G6PD mutation in diabetes patient was more in non-diabetes patient, (47) diabetes patient have Med-G6PD (78.3%) and (15) non diabetes have the mutation (15%) and the G6PD activity was significantly higher with Med-G6PD mutation in in diabetes patient (P<0.05) and non-significantly higher with non-diabetes patient. The Med-G6PD mutation can be used as molecular marker to diagnosis diabetes, Diabetic hyperglycemia may lead to serious complications and decrease G6PD activity. This issue itself aggravates diabetic injury due to inappropriate anti oxidation process. Simultaneous dyslipidemia and obesity may intensify the effect of hyperglycemia and oxidative stress.

Published in	Advances in Biochemistry (Volume 9, Issue 3)
DOI	10.11648/j.ab.20210903.17
Page(s)	82-89
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Glucose-6-phosphate Dehydrogenase (G6PD), Diabetes Mellitus, PCR-RFLP, Mediterranean Mutation

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Abdulqader Wael Rasheid, Firas Taher Maher, Akeel Hussein Al-Aisse, Adnan Fadhel Nasseef. (2021). Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Advances in Biochemistry, 9(3), 82-89. https://doi.org/10.11648/j.ab.20210903.17

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ACS Style

Abdulqader Wael Rasheid; Firas Taher Maher; Akeel Hussein Al-Aisse; Adnan Fadhel Nasseef. Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Adv. Biochem. 2021, 9(3), 82-89. doi: 10.11648/j.ab.20210903.17

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AMA Style

Abdulqader Wael Rasheid, Firas Taher Maher, Akeel Hussein Al-Aisse, Adnan Fadhel Nasseef. Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Adv Biochem. 2021;9(3):82-89. doi: 10.11648/j.ab.20210903.17

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@article{10.11648/j.ab.20210903.17,
  author = {Abdulqader Wael Rasheid and Firas Taher Maher and Akeel Hussein Al-Aisse and Adnan Fadhel Nasseef},
  title = {Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus},
  journal = {Advances in Biochemistry},
  volume = {9},
  number = {3},
  pages = {82-89},
  doi = {10.11648/j.ab.20210903.17},
  url = {https://doi.org/10.11648/j.ab.20210903.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20210903.17},
  abstract = {Diabetes mellitus is a metabolic disease belongs to a group of disparate diseases clinically and genetically characterized by increased blood sugar (hyperglycemia) as a result of defect in insulin metabolism. Glucose-6-Phosphate Dehydrogenase (G6PD) (Oxidoreductase, EC 1.1.1.1-49) is one of the most important enzymes of the metabolite, the main enzyme and the key to the Pentose phosphate path way. Unsuitable control of blood glucose decreases G6PD activity and increases diabetes mellitus complications. the This study evaluated effect of Mediterranean mutation which causes decrease G6PD activity on diabetes patients and evaluated the difference of G6PD activity among diabetic and non-diabetic patients, and the impact of hyperglycemia on the G6PD activity and different in G6PD activity in six and age and relation between Calcium and Magnesium with G6PD activity and Mediterranean mutation and found correlative coefficient(r) between G6PD activity in Patient and Blood sugar, Calcium, Magnesium and G6PD activity in healthy people. Asixteen diabetic and non-diabetic subjects were selected from patients 8 to 60 years old. Demographic data including gender, age, height, weight, duration of diabetes mellitus, type and duration of treatment, medical history (especially fauvism) were recorded. Blood pressure and body mass index were also measured. One blood sample was taken from each subject and 5 elements including G6PD presence and activity, fasting plasma glucose, plasma Calcium, Magnesium and DNA was extraction from whole blood and then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme MboII to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). G6PD activity was significantly higher in non-diabetic subjects (P The Med-G6PD mutation can be used as molecular marker to diagnosis diabetes, Diabetic hyperglycemia may lead to serious complications and decrease G6PD activity. This issue itself aggravates diabetic injury due to inappropriate anti oxidation process. Simultaneous dyslipidemia and obesity may intensify the effect of hyperglycemia and oxidative stress.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus
AU  - Abdulqader Wael Rasheid
AU  - Firas Taher Maher
AU  - Akeel Hussein Al-Aisse
AU  - Adnan Fadhel Nasseef
Y1  - 2021/08/31
PY  - 2021
N1  - https://doi.org/10.11648/j.ab.20210903.17
DO  - 10.11648/j.ab.20210903.17
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 82
EP  - 89
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20210903.17
AB  - Diabetes mellitus is a metabolic disease belongs to a group of disparate diseases clinically and genetically characterized by increased blood sugar (hyperglycemia) as a result of defect in insulin metabolism. Glucose-6-Phosphate Dehydrogenase (G6PD) (Oxidoreductase, EC 1.1.1.1-49) is one of the most important enzymes of the metabolite, the main enzyme and the key to the Pentose phosphate path way. Unsuitable control of blood glucose decreases G6PD activity and increases diabetes mellitus complications. the This study evaluated effect of Mediterranean mutation which causes decrease G6PD activity on diabetes patients and evaluated the difference of G6PD activity among diabetic and non-diabetic patients, and the impact of hyperglycemia on the G6PD activity and different in G6PD activity in six and age and relation between Calcium and Magnesium with G6PD activity and Mediterranean mutation and found correlative coefficient(r) between G6PD activity in Patient and Blood sugar, Calcium, Magnesium and G6PD activity in healthy people. Asixteen diabetic and non-diabetic subjects were selected from patients 8 to 60 years old. Demographic data including gender, age, height, weight, duration of diabetes mellitus, type and duration of treatment, medical history (especially fauvism) were recorded. Blood pressure and body mass index were also measured. One blood sample was taken from each subject and 5 elements including G6PD presence and activity, fasting plasma glucose, plasma Calcium, Magnesium and DNA was extraction from whole blood and then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme MboII to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). G6PD activity was significantly higher in non-diabetic subjects (P The Med-G6PD mutation can be used as molecular marker to diagnosis diabetes, Diabetic hyperglycemia may lead to serious complications and decrease G6PD activity. This issue itself aggravates diabetic injury due to inappropriate anti oxidation process. Simultaneous dyslipidemia and obesity may intensify the effect of hyperglycemia and oxidative stress.
VL  - 9
IS  - 3
ER  -

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Author Information

Abdulqader Wael Rasheid

Department of Chemistry, College of Science, University of Tikrit, Tikrit, Iraq
Firas Taher Maher

Department of Chemistry, College of Science, University of Tikrit, Tikrit, Iraq
Akeel Hussein Al-Aisse

Department of Biology, College of Science, University of Tikrit, Tikrit, Iraq
Adnan Fadhel Nasseef

Department of Biology, College of Science, University of Tikrit, Tikrit, Iraq

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Abdulqader Wael Rasheid, Firas Taher Maher, Akeel Hussein Al-Aisse, Adnan Fadhel Nasseef. (2021). Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Advances in Biochemistry, 9(3), 82-89. https://doi.org/10.11648/j.ab.20210903.17

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ACS Style

Abdulqader Wael Rasheid; Firas Taher Maher; Akeel Hussein Al-Aisse; Adnan Fadhel Nasseef. Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Adv. Biochem. 2021, 9(3), 82-89. doi: 10.11648/j.ab.20210903.17

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AMA Style

Abdulqader Wael Rasheid, Firas Taher Maher, Akeel Hussein Al-Aisse, Adnan Fadhel Nasseef. Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus. Adv Biochem. 2021;9(3):82-89. doi: 10.11648/j.ab.20210903.17

Copy | Download

@article{10.11648/j.ab.20210903.17,
  author = {Abdulqader Wael Rasheid and Firas Taher Maher and Akeel Hussein Al-Aisse and Adnan Fadhel Nasseef},
  title = {Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus},
  journal = {Advances in Biochemistry},
  volume = {9},
  number = {3},
  pages = {82-89},
  doi = {10.11648/j.ab.20210903.17},
  url = {https://doi.org/10.11648/j.ab.20210903.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20210903.17},
  abstract = {Diabetes mellitus is a metabolic disease belongs to a group of disparate diseases clinically and genetically characterized by increased blood sugar (hyperglycemia) as a result of defect in insulin metabolism. Glucose-6-Phosphate Dehydrogenase (G6PD) (Oxidoreductase, EC 1.1.1.1-49) is one of the most important enzymes of the metabolite, the main enzyme and the key to the Pentose phosphate path way. Unsuitable control of blood glucose decreases G6PD activity and increases diabetes mellitus complications. the This study evaluated effect of Mediterranean mutation which causes decrease G6PD activity on diabetes patients and evaluated the difference of G6PD activity among diabetic and non-diabetic patients, and the impact of hyperglycemia on the G6PD activity and different in G6PD activity in six and age and relation between Calcium and Magnesium with G6PD activity and Mediterranean mutation and found correlative coefficient(r) between G6PD activity in Patient and Blood sugar, Calcium, Magnesium and G6PD activity in healthy people. Asixteen diabetic and non-diabetic subjects were selected from patients 8 to 60 years old. Demographic data including gender, age, height, weight, duration of diabetes mellitus, type and duration of treatment, medical history (especially fauvism) were recorded. Blood pressure and body mass index were also measured. One blood sample was taken from each subject and 5 elements including G6PD presence and activity, fasting plasma glucose, plasma Calcium, Magnesium and DNA was extraction from whole blood and then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme MboII to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). G6PD activity was significantly higher in non-diabetic subjects (P The Med-G6PD mutation can be used as molecular marker to diagnosis diabetes, Diabetic hyperglycemia may lead to serious complications and decrease G6PD activity. This issue itself aggravates diabetic injury due to inappropriate anti oxidation process. Simultaneous dyslipidemia and obesity may intensify the effect of hyperglycemia and oxidative stress.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Effect of G6PD Activity, Mediterranean Mutation and Some Biomarkers on Patients with Diabetes Mellitus
AU  - Abdulqader Wael Rasheid
AU  - Firas Taher Maher
AU  - Akeel Hussein Al-Aisse
AU  - Adnan Fadhel Nasseef
Y1  - 2021/08/31
PY  - 2021
N1  - https://doi.org/10.11648/j.ab.20210903.17
DO  - 10.11648/j.ab.20210903.17
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 82
EP  - 89
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20210903.17
AB  - Diabetes mellitus is a metabolic disease belongs to a group of disparate diseases clinically and genetically characterized by increased blood sugar (hyperglycemia) as a result of defect in insulin metabolism. Glucose-6-Phosphate Dehydrogenase (G6PD) (Oxidoreductase, EC 1.1.1.1-49) is one of the most important enzymes of the metabolite, the main enzyme and the key to the Pentose phosphate path way. Unsuitable control of blood glucose decreases G6PD activity and increases diabetes mellitus complications. the This study evaluated effect of Mediterranean mutation which causes decrease G6PD activity on diabetes patients and evaluated the difference of G6PD activity among diabetic and non-diabetic patients, and the impact of hyperglycemia on the G6PD activity and different in G6PD activity in six and age and relation between Calcium and Magnesium with G6PD activity and Mediterranean mutation and found correlative coefficient(r) between G6PD activity in Patient and Blood sugar, Calcium, Magnesium and G6PD activity in healthy people. Asixteen diabetic and non-diabetic subjects were selected from patients 8 to 60 years old. Demographic data including gender, age, height, weight, duration of diabetes mellitus, type and duration of treatment, medical history (especially fauvism) were recorded. Blood pressure and body mass index were also measured. One blood sample was taken from each subject and 5 elements including G6PD presence and activity, fasting plasma glucose, plasma Calcium, Magnesium and DNA was extraction from whole blood and then amplified by polymerase chain reaction (PCR) and later subjected to digestion by restriction enzyme MboII to create restriction fragment length polymorphism (RFLP) to enable the detection of mutation that caused G6PD deficiency namely Mediterranean (Med). G6PD activity was significantly higher in non-diabetic subjects (P The Med-G6PD mutation can be used as molecular marker to diagnosis diabetes, Diabetic hyperglycemia may lead to serious complications and decrease G6PD activity. This issue itself aggravates diabetic injury due to inappropriate anti oxidation process. Simultaneous dyslipidemia and obesity may intensify the effect of hyperglycemia and oxidative stress.
VL  - 9
IS  - 3
ER  -

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